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  • 无菌室消毒灭菌操作要领!
    来源: 浏览: 发布日期:2019-10-25
    无菌室消毒
    Sterile room disinfection
    目的:建立无菌室清洁消毒的标准操作规程,为质量控制中心无菌室的清洁消毒提供标准,防止污染,保证药品质量控制与检验的准确性和真实性。
    Objective: to establish the standard operating procedures for the cleaning and disinfection of the sterile room in the quality control center, to prevent pollution and ensure the accuracy and authenticity of drug quality control and inspection.
    适用范围:适用于实验室无菌室的清洁消毒。质检中心无菌室工作人员负责本程序的实施,质保部负责监督。
    Scope of application: it is applicable to the cleaning and disinfection of the laboratory sterile room. The staff in the aseptic room of the quality inspection center is responsible for the implementation of this procedure, and the quality assurance department is responsible for the supervision.
    灭菌操作要点
    Key points of sterilization operation
    1. 首先对无菌室进行消毒,打开紫外线灯30-60分钟。
    1. First, sterilize the sterile room and turn on the ultraviolet lamp for 30-60 minutes.
    2. 用于检验的相关设备在进入无菌室前必须单独消毒。
    2. The relevant equipment for inspection must be sterilized separately before entering the sterile room.
    3.操作人员进入无菌室前,必须清洁消毒双手,穿戴无菌工作服、帽、鞋。
    3. Before entering the sterile room, operators must clean and disinfect their hands, and wear sterile work clothes, hats and shoes.
    4. 进入无菌室后,再次消毒双手,然后进行检查操作。
    4. After entering the sterile room, disinfect your hands again, and then carry out the inspection operation.
    操作过程记录
    Operation process record
    动作要轻,不要太快,以免搅动空气增加污染;玻璃器皿应轻拿轻放,以免损坏或污染环境。
    The action should be light, not too fast, so as not to stir the air to increase pollution; glassware should be handled with care, so as not to damage or pollute the environment.
    2. 操作应在火焰区域附近进行。
    2. The operation shall be carried out near the flame area.
    3.接种环、接种针等金属设备使用前、后均应烧灼。烧灼时应先经内火焰烘干残渣,再烧灼灭菌。
    3. Metal equipment such as inoculation ring and inoculation needle shall be burned before and after use. When cauterizing, the residue shall be dried by internal flame first, and then cauterized and sterilized.
    4. 使用吸管时,不要直接用嘴吸或吹吸管,要用耳珠操作。
    4. When using the pipette, do not directly use the mouth to suck or blow the pipette, but use the ear bead to operate.
    无菌室装修
    5. 观察盘子时最好不要把盖子打开。如果你想接触菌落进行检查,你必须在火焰附近操作。
    5. It is better not to open the lid when observing the plate. If you want to touch the colony for inspection, you must operate near the flame.
    6、对于可疑致病菌涂片染色,应使用夹片夹玻片,不要直接用手取玻片,以免造成污染,用过的玻片也应放在消毒剂中浸泡消毒,然后清洗。
    6. For the staining of the smear with suspected pathogenic bacteria, the slides should be sandwiched instead of being taken directly by hand to avoid pollution. The used slides should also be soaked in disinfectant for disinfection and then cleaned.
    7. 工作结束后,清理工作台上的样品和设备,用消毒剂擦拭工作台上。
    7. After the work, clean the samples and equipment on the worktable, and wipe the worktable with disinfectant.
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