1 、 sterilization in asepsis room. Before each use open above UV lamp 30min, or prior to use 30min on the inside and outside room 5% carbolic spray.
2, after washing with soap, the equipment needed to move into the outer chamber; in the outer room for sterilized working clothes, hats and shoes, wearing masks, and then use the 2% Lysol liquid dip hands for 2 minutes.
3, all kinds of necessities into check and chamber in place, with 5% in the working table and carbolic spray operator station space, return to the outer chamber, 5 ~ 10min after work into the chamber.
4, before inoculation, use 70% alcohol cotton ball to wipe hands; for aseptic operation, the action should be light and slow, as far as possible to reduce air fluctuation and ground dust.
5, work should pay attention to safety. In the case of cotton plug ignition, by hand or with a damp cloth wrapped out, do not blow through your mouth, in order to avoid the expansion of combustion; if there is a bacteria culture on bacteria or break the container, use carbolic cloth infiltration in 5% packages, and wipe with a cloth or table 5% carbolic infiltration ground, with rubbing alcohol hand before proceeding.
6, the work is over, immediately clean up the table, will not be stored in the asepsis room of the goods and waste all out of the asepsis room, the asepsis room with 5% carbolic spray, or ultraviolet light irradiation 30min.
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